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keratinocyte growth kit  (ATCC)


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    ATCC keratinocyte growth kit
    Keratinocyte Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/keratinocyte+growth+kit/Keratinocyte+Growth+Kit/pmc13084248-145-9-12
    Average 96 stars, based on 216 article reviews
    keratinocyte growth kit - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Epithelial cells fire voltage spikes.
    Article Snippet: .. Normal human epidermal keratinocytes (PCS200-010, ATCC) were cultured in Dermal Cell Basal Medium (PCS-200-030, ATCC) supplemented with the Keratinocyte Growth Kit (PCS-200-040, ATCC) at 37 °C with 5% CO2 to maintain their proliferation and inhibit undesired differentiation of keratinocytes. ..

    Article Title: Phosphatidylserine-Rich circulating extracellular vesicles activate TAM receptor signaling to promote skin wound repair.
    Article Snippet: Phosphatidylserine (PS) is an anionic phospholipid that acquires signaling activity when exposed on membrane surfaces.. Although small extracellular vesicles (sEVs) have been implicated in diverse biological processes, the contribution of membrane-associated PS to vesicle-mediated signaling remains incompletely understood.. Here, we show that circulating small extracellular vesicles (csEVs) activate dermal fibroblasts and keratinocytes through a PS-dependent receptor signaling mechanism.

    Article Title: KLS-3021: Innovative oncolytic virotherapy for the treatment of advanced primary and metastatic cutaneous squamous cell carcinoma
    Article Snippet: HSC-1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Welgene) supplemented with 20% FBS (Gibco), 1% antibiotic-antimycotic (Gibco), and 2 mM L-glutamine (Gibco). .. NHEKs were cultured in dermal cell basal medium with keratinocyte growth kit (ATCC) or keratinocyte growth medium 3 with supplement mix and 0.06 mM CaCl 2 (Promocell), according to the manufacturer’s instructions. .. Luciferase-labeled A-431 cells were cultured in DMEM (ATCC) supplemented with 10% FBS (Gibco), 1% penicillin-streptomycin (Sigma), and 8 μg/mL blasticidin (InvivoGen).

    Article Title: Phosphorylated lantibiotics-producing commensals integrate into the human oral microbiome to suppress pathogens and promote microbiome homeostasis.
    Article Snippet: .. TIGKs cells were cultured in a T75 Flask using Dermal Cell Basal Medium (ATCC PCS-200-030) supplemented with keratinocyte growth kit (ATCC PCS200-040) and pen-strep (50 I.U./mL penicillin and 50 μg/mL streptomycin) until they reached 80- 90% confluency. ..



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    keratinocyte growth medium ksfm  (PromoCell)
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    PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in <t>KSFM</t> medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).
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    PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in <t>KSFM</t> medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).
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    Image Search Results


    PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).

    Journal: PLOS One

    Article Title: Fatty acid-binding protein 5 (FABP5) modulates limbal epithelial cell homeostasis by regulating the expression of key genes under both normal and inflammatory conditions , in vitro

    doi: 10.1371/journal.pone.0347228

    Figure Lengend Snippet: PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).

    Article Snippet: After centrifugation at 200g for 5 minutes, cells were cultured in Keratinocyte growth medium (KSFM) (Cat. Nr. C-20111, Promocell, Heidelberg, Germany), at 37 oC, 5% CO 2 .

    Techniques: Expressing, Transfection, Control, Western Blot, Knockdown